I-EZER NAAT SARS-CoV-2 ye-RT-PCR Kit yesikhathi sangempela
Isingeniso
I-EZERTMI-SARS-CoV-2 NAAT (i-SARS-CoV-2 yesikhathi sangempela i-RT-PCR Kit) isetshenziselwa ukutholwa kwe-in vitro qualitative ye-novel coronavirus SARS-CoV-2 ezibonelweni zokuphefumula ezihlanganisa i-swab yomphimbo kanye ne-nasopharyngeal swab.Amasethi e-Primer kanye ne-FAM enelebuli yophenyo yakhelwe ukutholwa okuthile kofuzo lwe-ORFlab lwe-SARS-CoV-2, i-VIC enelebuli yophenyo lwe-N gene ye-SARS-CoV-2.I-Human RNase Pgene ekhishwe kanyekanye nesampula yokuhlola inikeza ukulawula kwangaphakathi ukuze kuqinisekiswe inqubo yokukhipha i-nucleic kanye nobuqotho be-reagent.Uphenyo oluqondiswe kufuzo lomuntu lwe-RNase P lubhalwe ukuthi CY5.
Ama-coronavirus anoveli awohlobo lwe-β.I-SARS-CoV-2 yisifo esithathelwanayo sokuphefumula esinamandla.Ngokuvamile abantu bayathinteka.Njengamanje, iziguli ezingenwe yi-coronavirus yenoveli ziwumthombo oyinhloko wokutheleleka;abantu abane-asymptomatic abangenwe yilesi sifo nabo bangathathelwana.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezi-1 kuya kweziyi-14, izikhathi eziningi izinsuku ezi-3 kuya kweziyi-7.Izimpawu eziyinhloko zihlanganisa umkhuhlane, ukukhathala nokukhwehlela okomile.Ukucinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo kutholakala ezimweni ezimbalwa.
UKUPHATHWA KOMHLABA KANYE NOSONDO
• Izibonelo Ezivumelekile: i-nasopharyngeal swab kanye ne-throat swab.
• Qoqa amasampula kumashubhu angenalutho.
• Ukungcola kufanele kugwenywe ngesikhathi sokuqoqwa, ukugcinwa kanye nokuthuthwa kwamasampula.
• Wonke amasampula aqoqiwe kufanele athathwe njengathelelanayo futhi angasebenzi ku-56℃imizuzu engama-30.
• Izibonelo zingagcinwa ku-2-8℃ kuze kufike emahoreni angama-24 ngemva kokuqoqwa futhi ku--70℃ noma ngaphansi ukuze zilondolozwe isikhathi eside.Gwema umjikelezo wokuqandisa ophindaphindiwe wesampula futhi uqinisekise ukuthi isampula incibilika ngokuphelele ngaphambi kokukhipha i-RNA.
• Amasampula okuthutha ebhokisini elivaliwe elineqhwa elomile noma isikhwama seqhwa.
Izimo zokugcina ikhithi
Le kit kufanele igcinwe ku-2 ℃ ~ 30 ℃ futhi ekuvimbeleni ukukhanya.
Ama-reagents e-Freeze-Dried afakwe nge-vacuum futhi avaliwe esikhwameni se-aluminium foil.Ngemuva kokuvula, sicela ugcine imikhiqizo engasetshenziswanga esikhwameni sepulasitiki esikwazi ukuvala kabusha esinama-desiccants, ukhame umoya, bese ubuyisela esikhwameni se-aluminium foil.Ukulawula Okuhle kufanele kugcinwe ku -20±5 ℃ ngemva kokwakhiwa kabusha.
Okuqukethwe
| Izingxenye | Qty |
| Ingxube ye-RT-PCR eneziqephu ezingu-8 | 8-strip ishubhu× 6 |
| Ukulawula Okuhle okomisiwe | 1 ithubhu |
| Ukulawula Okungalungile | 100 μL × 1 ishubhu |
| Isithameli Sokumisa kabusha | 100 μL × 1 ishubhu |
| I-PCR 8-strip Cap yesikhathi sangempela | 8-strip ishubhu× 6 |
| Faka iphakheji | 1 ngakunye |
Ingxenye #:N211101 EZERTMI-SARS-CoV-2 NAAT (Kulula)
| Izingxenye | Qty |
| Ingxube ye-RT-PCR efriziwe | 1 ibhodlela |
| Ukulawula Okuhle okomisiwe | 1 ithubhu |
| Ukulawula Okungalungile | 100 μL × 1 ishubhu |
| Isithameli Sokumisa kabusha | 100 μL × 1 ishubhu |
| Faka iphakheji | 1 ngakunye |
Ingxenye #:N211102 EZERTMI-SARS-CoV-2 NAAT (Inqwaba)
Uku-oda Ulwazi
| Umkhiqizo | Ikati.No. | Okuqukethwe |
| Ikhithi ye-RT-PCR yesikhathi sangempela yakwa-SARS-CoV-2 (Kulula) | I-N211101 | 48 Izivivinyo |
| Ikhithi ye-RT-PCR yesikhathi sangempela yakwa-SARS-CoV-2 (Inqwaba) | I-N211102 | 48 Izivivinyo |
